产品信息
反相HPLC条件下常用缓冲盐与添加剂
反相HPLC条件下常用缓冲盐与添加剂
种类 | pKa | Buffer range | 常用浓度 | 挥发性 (LC/MS、制备的适用性) |
TFA | <1.0 | 0.01~0.1% | ○ | |
H3PO4 | 2.1 | 0.01~0.1% | × | |
NH4H2PO4-H3PO4 [NaH2PO4-H3PO4, KH2PO4-H3PO4] |
2.1 | 1.1~3.1 | 5~50 mM (推荐<20 mM) |
× |
HCOOH | 3.7 | 0.1~1.0% | ○ | |
HCOONH4-HCOOH [HCOONa-HCOOH, HCOOK-HCOOH] |
3.7 | 2.7~4.7 | 5~50 mM | NH4 salt:○ [Na, K salt:×] |
CH3COOH | 4.8 | 0.5~5.0% | ○ | |
CH3COONH4-CH3COOH [CH3COONa-CH3COOH, CH3COOK-CH3COOH] |
4.8 | 3.8~5.8 | 5~50 mM | NH4 salt:○ [Na, K salt:×] |
(NH4)2HPO4 -NaH2PO4 [Na2HPO4-NaH2PO4, K2HPO4-KH2PO4] |
7.2 | 6.2~8.2 | 5~50 mM (推荐<20 mM) |
× |
Triethylamine-CH3COOH (TEAA) | 4.6~6, 10~11 | <20 mM | ○ | |
HCOONH4-NH3, CH3COONH4-NH3 | 9.2 | 8.2~10.2 | <20 mM | ○ |
Na3PO4-Na2HPO4, K3HPO4-K2HPO4 | 12.3 | 11.3~13.3 | <10 mM | × |
NH4HCO3-NH3 | 8.5~10.5 | <10 mM | ○ |