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逆相HPLCで使用される主な緩衝液や添加剤

逆相HPLCで使用される主な緩衝液や添加剤

種類 pKa Buffer range 一般的な使用濃度 揮発性
(LC/MSや分取への適応)
TFA <1.0   0.01~0.1%
H3PO4 2.1   0.01~0.1% ×
NH4H2PO4-H3PO4
[NaH2PO4-H3PO4, KH2PO4-H3PO4]
2.1 1.1~3.1 5~50 mM
(<20 mM推奨)
×
HCOOH 3.7   0.1~1.0%
HCOONH4-HCOOH
[HCOONa-HCOOH, HCOOK-HCOOH]
3.7 2.7~4.7 5~50 mM NH4 salt:○
[Na, K salt:×]
CH3COOH 4.8   0.5~5.0%
CH3COONH4-CH3COOH
[CH3COONa-CH3COOH, CH3COOK-CH3COOH]
4.8 3.8~5.8 5~50 mM NH4 salt:○
[Na, K salt:×]
(NH4)2HPO4 -NaH2PO4
[Na2HPO4-NaH2PO4, K2HPO4-KH2PO4]
7.2 6.2~8.2 5~50 mM
(<20 mM推奨)
×
Triethylamine-CH3COOH (TEAA)   4.6~6, 10~11 <20 mM
HCOONH4-NH3, CH3COONH4-NH3 9.2 8.2~10.2 <20 mM
Na3PO4-Na2HPO4, K3HPO4-K2HPO4 12.3 11.3~13.3 <10 mM ×
NH4HCO3-NH3   8.5~10.5 <10 mM

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